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primary normal human melanocyte (nhem) cells ![]() Primary Normal Human Melanocyte (Nhem) Cells, supplied by DS Pharma Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/normal+human+melanocytes+nhem/pmc04239619-172-0-19?v=DS+Pharma+Biomedical Average 90 stars, based on 1 article reviews
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Cell pellet of NHEM from the epidermis of juvenile foreskin in RNAlater® for subsequent RNA, DNA or protein analysis. Cell pellet consisting of 1 million cells dissolved in 200 µl RNAlater® for subsequent RNA, DNA
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Primary human epidermal melanocytes isolated in PMA-free Melanocyte Growth Medium M3. Available from juvenile and adult donors. Primary Normal Human Epidermal Melanocytes M3 (NHEM M3) are isolated from the epidermis of juvenile foreskin or adult
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Primary human epidermal melanocytes isolated in PMA-free Melanocyte Growth Medium M3. Available from juvenile and adult donors. Primary Normal Human Epidermal Melanocytes M3 (NHEM M3) are isolated from the epidermis of juvenile foreskin or adult
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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: 67-kDa Laminin Receptor-dependent Protein Phosphatase 2A (PP2A) Activation Elicits Melanoma-specific Antitumor Activity Overcoming Drug Resistance
doi: 10.1074/jbc.M114.604983
Figure Lengend Snippet: EGCG-induced PP2A activation regained the sensitivity of BRAF inhibitor (PLX4720). A, phosphorylation level and expression of P70S6k and S6 were analyzed by Western blot analysis. B, melanoma cells were treated with PLX4720 of for 48 h, and the phosphorylation level of P70S6k, S6 and ERK1/2 were analyzed by Western blot analysis. C, Hs294T cells were treated with EGCG for 24 h, and the activity of PP2A was measured. D, Hs294T cells were treated with EGCG for 96 h in the presence or absence of OA. E, Hs294T cells were treated with EGCG (5 μm) of and the phosphorylation levels of P70S6k and S6 were analyzed by Western blot analysis. F, Hs294T cells were treated with EGCG for 48 h in the presence or absence of OA, and the phosphorylation levels of P70S6k and S6 were measured. G, Hs294T cells were treated with EGCG, PLX4720, or EGCG/PLX4720 in combination for 96 h. H, NHEM were treated with EGCG or rapamycin (Rapa.) alone or combined with PLX4720 for 96h. I, Hs294T cells were treated with EGCG or rapamycin for 24 h. J, Hs294T cells were treated with EGCG, rapamycin, or PLX4720 alone, or in combination for 14 days for long term colony formation assay. K–N, effect of PLX4720 and EGCG combination on the tumor growth (K), tumor weight (L), p70S6k and ERK phosphorylation (n = 4) (M), aspartate transaminase (AST) and alanine aminotransferase (ALT) (N) of mice on day 33 after inoculation. O, signaling pathway schematic. Error bars, S.D. in vitro or S.E. in vivo (n = 3 per group in vitro or n = 7 per group in vivo). *, p < 0.05; **, p < 0.01; *** or ###, p < 0.001. DMSO, dimethyl sulfoxide; n.s., not significant.
Article Snippet:
Techniques: Activation Assay, Phospho-proteomics, Expressing, Western Blot, Activity Assay, Colony Assay, In Vitro, In Vivo